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Journal : Agroista: Jurnal Agroteknologi

DETEKSI KOMUNITAS BAKTERI PADA DIAPHORINA CITRI JANTAN DAN BETINA, VEKTOR PENYAKIT HUANGLONGBING PADA JERUK MENGGUNAKAN PCR-RISA Achmad Himawan; Siti Subandiyah
AGROISTA : Jurnal Agroteknologi Vol. 1 No. 1 (2017): MEI
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.755 KB) | DOI: 10.55180/agi.v1i1.5

Abstract

Huanglongbing (HLB) is a very destructive disease on citrus. Diaphorina citri is a HLB disease vector. The aim of this research is to detec bacterial community in the insect vector, especially D. citri males and females, using Polimerase Chain Reaction-Ribosomal Intergenic Spacer Analysis (PCR-RISA) method. The results of research show that there are 3 – 4 bacteria species in D. citri males and 1 – 4 bacteria species in D. citri females.
DETEKSI BAKTERI RHIZOSFER KELAPA SAWIT DI TANAH MINERAL MENGGUNAKAN METODE PCR-RISA Achmad Himawan; Hangger Gahara Mawandha
AGROISTA : Jurnal Agroteknologi Vol. 2 No. 1 (2018): MEI
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.918 KB) | DOI: 10.55180/agi.v2i1.29

Abstract

Oil palm (Elaeis guineensis Jack.) plantation is the biggest plantation in Indonesia. Indonesia is the biggest Crude Palm Oil (CPO) in the world since 2017. To maintain oil palm productivity is needed soil fertility research, especially rhizosphere bacteria. The aim of this research is to detect rhizosphere bacteria in mineral soil at oil palm plantation in Riau. Detection the bacteria used Polimerase Chain ReactionRibosomal Intergenic Spacer Analysis (PCR-RISA) method. The research result showed that there is one bacterium species in the mineral soil. Keywords: rhizosphere bacteria, oil palm, mineral soil, PCR-RISA
APLIKASI CENDAWAN ENTOMOMOPATOGEN Cordyceps militaris PADA HAMA ULAT API (Setothosea asigna) DI PERKEBUNAN SATYA KISMA USAHA UNIT BATANG GADING MUARA BUNGO JAMBI Budi Purnama; Achmad Himawan; Samsuri Tarmadja
AGROISTA : Jurnal Agroteknologi Vol. 5 No. 2 (2021): NOVEMBER
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.406 KB) | DOI: 10.55180/agi.v5i2.103

Abstract

The aims of this research are to determine the best medium to propagation entomopathogenic fungi C. militaris, to determine effectivity and dosage of C. militaris to control pupae of S. asigna. This research was conducted in plantation of PT. Satya Kisma Usaha Unit Batang Gading, Tanah Tumbuh County, Muara Bungo District, Jambi. The research was conducted from August to September 2020. This study used a non-factorial method, which was arranged in a completely randomizing design (CRD) with 1 factor, namely growth media. The growth media are consisted of corn seeds (M1) and bran (M2) and repeated 5 times. For dosage of fungi used a non-factorial method, which was arranged in a CRD with 1 factor, namely bran media. The bran media dosages are P0 = control (100 ml aquadest/polybag), P1 = 10 g C. militaris + 100 ml aquadest/polybag, P2 = 20 g C. militaris + 100 ml aquadest/polybag, P3 = 30 g C. militaris + 100 ml aquadest/polybag P4 = 40 g C. militaris + 100 ml aquadest/polybag and all of them are repeated 4 times. The results showed that bran medium is the best medium for fungi propagation. C. militaris was effectively to control pupae of S. asigna. The best of fungi dosage is P4 = 40 g C. militaris + 100 ml aquadest/polybag. The highest pupae mortality (100%) is achieved at 12 days observation. Keywords: entomopathogenic fungi, Cordyceps militaris, bran, Setothosea asigna